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elabscience e el h 0654  (Elabscience Biotechnology)


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    Elabscience Biotechnology elabscience e el h 0654
    Elabscience E El H 0654, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cartilage+oligomeric+matrix+protein+comp/Human+COMP+(Cartilage+Oligomeric+Matrix+Protein)+ELISA+Kit/pmc12767245-18-3-3
    Average 93 stars, based on 8 article reviews
    elabscience e el h 0654 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Sandwich ELISA:

    Article Title: Post‐operative injection of hydrolyzed collagen peptides shows anti‐inflammatory effect in patients with femoroacetabular impingement improving the early recovery
    Article Snippet: Vascular endothelial growth factor (VEGF), a potent mediator of both angiogenesis and vasculogenesis, a marker of inflammation, was also detected in SFs with a sandwich enzyme‐linked assay (ELISA) Kit (Human VEGF Quantikine ELISA Kit, R&D Systems, Inc.). .. Two sensitive markers of cartilage degradation in OA, C‐telopeptide fragments of type II collagen (CTX‐II) and Cartilage Oligomeric Matrix Protein (COMP), were evaluated in SFs and urine using commercially available Sandwich‐ELISA Kits (Elabscience Biotechnology Inc.). .. The kit standard curves were fit with a four parameters logistic curve, based on these curves, the unknown concentrations were calculated starting from the optical densities red with a microplate reader Infinite® M Plex (Tecan Trading AG) (the equations used are described in Supporting Information S1: Table ).

    Incubation:

    Article Title: Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?
    Article Snippet: Antigen retrieval step was performed by 10 min enzymatic retrieval with proteinase K (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C. .. Swine-specific primary antibodies targeting osteocalcin—OC (Abcam, Cambridge, UK, dilution 1:100), glucocorticoid receptor—GR (Santa Cruz Biotechnology, Santa Cruz, CA, USA, dilution 1:50) osteoprotegerin—OPG (Abcam, Cambridge, UK, dilution 1:100), receptor activator of nuclear factor kappa-Β ligand—RANKL (Biorbyt, USA, dilution 1:50), tissue inhibitor of metalloproteinases 2—TIMP-2 (Abcam, Cambridge, UK, dilution 1:100), bone morphogenetic protein 2—BMP-2 (Abcam, Cambridge, UK, dilution 1:250), vascular endothelial growth factor—VEGF (Biorbyt Ltd., Cambridge, UK, dilution 1:50), and cartilage oligomeric matrix protein—COMP (Elabscience, USA, dilution 1:100) were incubated overnight at 4 °C in blocking buffer (TBS with 5% goat serum; Sigma-Aldrich, St. Louis, MO, USA). .. The next day, peroxidase conjugated goat anti-rabbit (Rockland Immunochemicals, Inc. Pottstown, PA, USA, dilution 1:500) secondary antibody was incubated for 30 min.

    Blocking Assay:

    Article Title: Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?
    Article Snippet: Antigen retrieval step was performed by 10 min enzymatic retrieval with proteinase K (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C. .. Swine-specific primary antibodies targeting osteocalcin—OC (Abcam, Cambridge, UK, dilution 1:100), glucocorticoid receptor—GR (Santa Cruz Biotechnology, Santa Cruz, CA, USA, dilution 1:50) osteoprotegerin—OPG (Abcam, Cambridge, UK, dilution 1:100), receptor activator of nuclear factor kappa-Β ligand—RANKL (Biorbyt, USA, dilution 1:50), tissue inhibitor of metalloproteinases 2—TIMP-2 (Abcam, Cambridge, UK, dilution 1:100), bone morphogenetic protein 2—BMP-2 (Abcam, Cambridge, UK, dilution 1:250), vascular endothelial growth factor—VEGF (Biorbyt Ltd., Cambridge, UK, dilution 1:50), and cartilage oligomeric matrix protein—COMP (Elabscience, USA, dilution 1:100) were incubated overnight at 4 °C in blocking buffer (TBS with 5% goat serum; Sigma-Aldrich, St. Louis, MO, USA). .. The next day, peroxidase conjugated goat anti-rabbit (Rockland Immunochemicals, Inc. Pottstown, PA, USA, dilution 1:500) secondary antibody was incubated for 30 min.

    Concentration Assay:

    Article Title: Obacunone acts as a histone deacetylase 1 inhibitor to limit p38MAPK signaling and alleviate osteoarthritis progression
    Article Snippet: .. The concentration of cartilage oligomeric matrix protein (COMP) in the supernatant of serum was measured using an enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, Wuhan, China). .. Tissue samples were sliced to 4 μm thickness and stained using a Safranin O and Fast Green Cartilage staining kit (Beyotime, Shanghai, China) or HE staining kit (Sangon Biotech, Shanghai, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Obacunone acts as a histone deacetylase 1 inhibitor to limit p38MAPK signaling and alleviate osteoarthritis progression
    Article Snippet: .. The concentration of cartilage oligomeric matrix protein (COMP) in the supernatant of serum was measured using an enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, Wuhan, China). .. Tissue samples were sliced to 4 μm thickness and stained using a Safranin O and Fast Green Cartilage staining kit (Beyotime, Shanghai, China) or HE staining kit (Sangon Biotech, Shanghai, China).

    other:

    Article Title: Effects of Palm Tocotrienol-Rich Fraction Alone or in Combination with Glucosamine Sulphate on Grip Strength, Cartilage Structure and Joint Remodelling Markers in a Rat Model of Osteoarthritis
    Article Snippet: The serum level of cartilage oligomeric matrix protein (COMP) (Cat: E-EL-R0159, Elabscience, Wuhan, China) was measured using an enzyme-linked immunosorbent assay kit following the manufacturer’s instruction.



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    Image Search Results


    Scheme 1 Scheme of the classical QLISA detection method. COMP (cartilage oligomeric matrix protein) and hGH (human growth hormone) proteins are attached to the bottom of the plate, incubated with biotinylated detection antibodies, and then conjugated with streptavidin-coated QDs, followed by the direct registration of fluorescence signal from the bottom of the plate.

    Journal: International Journal of Nanomedicine

    Article Title: Development of a Sensitive Quantum Dot-Linked Immunoassay for the Multiplex Detection of Biochemical Markers in a Microvolumeric Format

    doi: 10.2147/ijn.s477118

    Figure Lengend Snippet: Scheme 1 Scheme of the classical QLISA detection method. COMP (cartilage oligomeric matrix protein) and hGH (human growth hormone) proteins are attached to the bottom of the plate, incubated with biotinylated detection antibodies, and then conjugated with streptavidin-coated QDs, followed by the direct registration of fluorescence signal from the bottom of the plate.

    Article Snippet: Prior to the experiment, the gold surface of the SPR sensor disc (SD AU, XanTec bioanalytics GmbH, Münster, Germany) was cleaned, modified with a self-assembled monolayer of 11-mercaptoundecanoic acid (Sigma-Aldrich, Steinheim, Germany) and capture antibodies against COMP (monoclonal mouse IgG1 clone 16F12, BioVendor, Brno, Czech Republic; Cat. No. RD182080100F1-01) were immobilized.

    Techniques: Incubation, Fluorescence

    Scheme 2 Singleplex detection of an analyte in microvolume format. Formation of COMP (cartilage oligomeric matrix protein), or hGH (human growth hormone) complexes with biotin-labeled anti-COMP or anti-hGH antibodies, respectively, and streptavidin-coated QDs of different spectra. Immune-complex disassembling solution dissociates the complexes through an analyte-biotinylated antibody connection. After dissociation, microdrops are transferred to microvolume reusable glass slide-based spectrophotometric scan.

    Journal: International Journal of Nanomedicine

    Article Title: Development of a Sensitive Quantum Dot-Linked Immunoassay for the Multiplex Detection of Biochemical Markers in a Microvolumeric Format

    doi: 10.2147/ijn.s477118

    Figure Lengend Snippet: Scheme 2 Singleplex detection of an analyte in microvolume format. Formation of COMP (cartilage oligomeric matrix protein), or hGH (human growth hormone) complexes with biotin-labeled anti-COMP or anti-hGH antibodies, respectively, and streptavidin-coated QDs of different spectra. Immune-complex disassembling solution dissociates the complexes through an analyte-biotinylated antibody connection. After dissociation, microdrops are transferred to microvolume reusable glass slide-based spectrophotometric scan.

    Article Snippet: Prior to the experiment, the gold surface of the SPR sensor disc (SD AU, XanTec bioanalytics GmbH, Münster, Germany) was cleaned, modified with a self-assembled monolayer of 11-mercaptoundecanoic acid (Sigma-Aldrich, Steinheim, Germany) and capture antibodies against COMP (monoclonal mouse IgG1 clone 16F12, BioVendor, Brno, Czech Republic; Cat. No. RD182080100F1-01) were immobilized.

    Techniques: Labeling

    Figure 3 Optimization of COMP analyte detection on the microslide. Non-diluted and diluted 1:1 (v/v) with PBS 10 mM NaOH and 0.5% SDS buffer on a glass microslide. At concentrations 50 nM, 25 nM, and 5 nM COMP was analyzed using 17C10 anti-COMP-biotinylated Ab and streptavidin-coated QD655. MFI – median fluorescence intensity; Blank – sample without COMP protein coating, followed by all of the other protocol steps. P-values indicate statistical significance (***P ≤ 0.001). Error bars represent the standard deviation of the average value (N=3).

    Journal: International Journal of Nanomedicine

    Article Title: Development of a Sensitive Quantum Dot-Linked Immunoassay for the Multiplex Detection of Biochemical Markers in a Microvolumeric Format

    doi: 10.2147/ijn.s477118

    Figure Lengend Snippet: Figure 3 Optimization of COMP analyte detection on the microslide. Non-diluted and diluted 1:1 (v/v) with PBS 10 mM NaOH and 0.5% SDS buffer on a glass microslide. At concentrations 50 nM, 25 nM, and 5 nM COMP was analyzed using 17C10 anti-COMP-biotinylated Ab and streptavidin-coated QD655. MFI – median fluorescence intensity; Blank – sample without COMP protein coating, followed by all of the other protocol steps. P-values indicate statistical significance (***P ≤ 0.001). Error bars represent the standard deviation of the average value (N=3).

    Article Snippet: Prior to the experiment, the gold surface of the SPR sensor disc (SD AU, XanTec bioanalytics GmbH, Münster, Germany) was cleaned, modified with a self-assembled monolayer of 11-mercaptoundecanoic acid (Sigma-Aldrich, Steinheim, Germany) and capture antibodies against COMP (monoclonal mouse IgG1 clone 16F12, BioVendor, Brno, Czech Republic; Cat. No. RD182080100F1-01) were immobilized.

    Techniques: Fluorescence, Standard Deviation

    Figure 4 Different COMP concentration (50 nM to 3.125 nM) detection in 96-well plate vs microvolumetric format. 17C10 anti-COMP-biotinylated Ab and streptavidin- coated QD655 were used. Purple bars indicate the signal detected from the bottom of the plate, excitation 400 nm, emission 655 nm, 80 μL volume. Blue bars indicate the signal detected on a microvolume glass slide (2 µL/sample), excitation 400 nm, emission 655 nm. Blank – every protocol step followed, excluding coating the bottom of the well with COMP (non-specific signal). P-values indicate statistical significance (****P < 0.0001). Error bars represent the standard deviation of the average value (96-well plate N=2; microvolumes N=3).

    Journal: International Journal of Nanomedicine

    Article Title: Development of a Sensitive Quantum Dot-Linked Immunoassay for the Multiplex Detection of Biochemical Markers in a Microvolumeric Format

    doi: 10.2147/ijn.s477118

    Figure Lengend Snippet: Figure 4 Different COMP concentration (50 nM to 3.125 nM) detection in 96-well plate vs microvolumetric format. 17C10 anti-COMP-biotinylated Ab and streptavidin- coated QD655 were used. Purple bars indicate the signal detected from the bottom of the plate, excitation 400 nm, emission 655 nm, 80 μL volume. Blue bars indicate the signal detected on a microvolume glass slide (2 µL/sample), excitation 400 nm, emission 655 nm. Blank – every protocol step followed, excluding coating the bottom of the well with COMP (non-specific signal). P-values indicate statistical significance (****P < 0.0001). Error bars represent the standard deviation of the average value (96-well plate N=2; microvolumes N=3).

    Article Snippet: Prior to the experiment, the gold surface of the SPR sensor disc (SD AU, XanTec bioanalytics GmbH, Münster, Germany) was cleaned, modified with a self-assembled monolayer of 11-mercaptoundecanoic acid (Sigma-Aldrich, Steinheim, Germany) and capture antibodies against COMP (monoclonal mouse IgG1 clone 16F12, BioVendor, Brno, Czech Republic; Cat. No. RD182080100F1-01) were immobilized.

    Techniques: Concentration Assay, Standard Deviation